This means that it is an intracellular enzyme. Oxygen is soluble in water, but dissolves only slowly in water at normal room temperatures. How does pH affect the reaction rate of catalase? This principle is based on something called the collision theory. Calculate the distance of the water movement at each concentration of hydrogen peroxide. Let the water cool to 80°C using thermometer.
When the potato is cut into eights I predict that there would be a faster reaction occurring as now the potato is doubled there would be plenty of surface area within the potato and the collision would be doubled due to the amount being cut to eights. In this lab the enzyme concentration is increased to see its effect. Because we were only able to stimulate an environment of a lower pH, we were only able to get 1 direction of slope, showing linear results. Flip the bottle so the hydrogen peroxide mixes with the catalase filter paper. After three minutes was over I repeated the experiment keeping everything the same except this time I used 2g of crushed liver instead of potato.
But there are certain conditions that need to be fulfilled in order for. In this experiment, the pHs of the buffer solutions will be varied, as shown in the concentrations table above. Volume could be measured instead in height using gas Syringe which will give more accurate results 3. It therefore competes with the substrate for the active site, so the reaction is slower. Problem What happens when a potato is combined with hydrogen peroxide?. The substrate in use will be Hydrogen Peroxide and we will be testing to find out how increasing the concentration of this will affect its reaction with the enzyme.
To make sure that I was making my experiment as safe as possible, I had to do a risk assessment of my experiment. Catalase, Catalysis, Chemical equilibrium 1053 Words 4 Pages Function of Enzymes Rena Mototani Glen Rock High School Advanced Biology 2014-2015 Effects of pH on the Function of Catalase Rena Mototani Problem: How does the pH of a cell affect the function of the enzyme catalase? The methodology includes procedures; multiple variables were tested in specific concentrations; that test the reaction rates of the enzyme catalase over a fixed period of time. Feel the temperature of the test tube with your hand. The laboratory was also conducted in association with a second laboratory that measured the effects of an inhibitor on the enzymes. However if the cells have a small surface area then the reaction rate will be slow because less of the nzyme will be accessible to the product and therefore fewer products will be made in a certain amount of time.
The concentration of the reactant will affect the reaction rate. Buffer solution, Celsius, Chemical reaction 1403 Words 5 Pages Temperature Affecting Enzyme Activity Introduction The basic properties of life revolve around chemical reactions. Also, the reaction is so vigorous that bubbles of mixture can carry pieces of liver into the delivery tube. Reassemble the apparatus, refill the measuring cylinder, and repeat from g to j with another concentration of hydrogen peroxide. The active sit of an enzyme is the part of the enzyme that determines what the enzyme will react with. This enzyme is called catalase.
If I was to do the experiment with a high concentration the particles are crowed close together and therefore it would collide with each other more often, which will give me a result of successful collisions. Catalase is found in many living tissues of organisms. Hypothesis: I think that the reaction rate for the liver will be faster than the reaction rate of the potato. Catalase is such an anti-oxidant molecule. There will have been limitations in the results as the range of buffer solution pHs used in the experiment were inadequate to determine an exact optimal pH for the enzyme Catalase to function.
In fact, your cells are always making poisonous chemicals. Remove the test tube from the hot water bath, allow it to air cool, then pour out the water. Introduction: In anaerobic conditions yeast cells break down sugar molecules into ethanol and produce carbon dioxide. Enzymes are proteins that are used to speed up these reactions without being consumed by them Mader, 2010. Suck a small amount of distilled water ~1ml into the pipette with the help of pipette filler and mark the initial position of the water.
Then I measured out 20 ml of hydrogen peroxide and diluted it with 20 ml of water making a 50% concentration solution of hydrogen peroxide. Enzymes are basically proteins, which have specific shapes for different substrates. Reduced concentration of substrate available 4. So enzymes are never wasted and always recycled. I can tell that this is true form my graph because the line for the liver reaction rate is steeper that the line for the potato reaction rate. I would take this experiment further by finding out if other organisms held more catalase. One reaction turns the hydrogen peroxide into water and oxygen.
This is because plants are very inactive and do not produce a lot of hydrogen peroxide and therefore do not need to have a lot of catalase to get rid of it. Our results showed that as the concentration of H2O2 increased, the rate of reaction increased. Background Information Enzymes such as Catalase are protein molecules which are found in living cells. It might seem strange to use dead cells to study the function of enzymes. Results When the substrate of hydrogen peroxide was added to the substances each performed a vastly different reaction.
At a high temperature there will be more particles colliding with enough energy to make the reaction happen. Our experiment had shown this reaction accurately. After adding my potato to the beaker I added the hydrogen peroxide and recorded the weight in my table. By adding some ice to the water, the temperature of beaker 1 is brought to 15°C and beaker 2 to 20°C. I would also repeat my experiment more so that I could get a more accurate result.