Physical methods of sterilization in microbiology. Using Physical Methods to Control Microorganisms 2019-01-05

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FAQ: Physical and Chemical Control of Microorganisms

physical methods of sterilization in microbiology

So of the available methods, 1. One of the oldest forms of sterilization is. They are designed to withstand high heat, high salt, and low nutrition conditions. Here thermostable materials on the racks inside the hot air oven. It consists of a pad of organic compounds such as cellulose acetate or polycarbonate, mounted in a holding device.

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Sterilization physical methods

physical methods of sterilization in microbiology

Rubber materials and inflammable materials should not be kept inside. It has many uses: Incinerators are used to carry out this process and are regularly employed in hospitals and research labs to destroy hospital and laboratory wastes. The saturated steam is formed at boiling temperature of water, i. However, most sonic machines are not reliable for regular use in disinfection or sterilization. Auto clave is used to sterilize equipment that does not get burnt and can withstand high temperatures, like powder or glass ware.

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Sterilization physical methods

physical methods of sterilization in microbiology

One can readily purchase active dried yeast for baking purposes and after the addition of water, the yeasts begin to carry out active metabolism. The benefits of this method are that it is incredibly effective for not only killing bacteria but also destroying viruses and vegetative spores that might persist in other control methods. Almost immediately, he encounters an epidemic of typhoid fever. What are some possible disadvantages of doing so? One example is discussed in MicroFocus. Heat is fast, reliable, and relatively inexpensive, and it does not introduce chemicals to a substance, as disinfectants sometimes do. Glass slides, scalpels, and mouths of culture tubes are sterilized by passing them through the Bunsen flame without allowing them to become red hot.

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Physical Methods of Control

physical methods of sterilization in microbiology

A brief description of the mode of action of each class of chemical disinfectant is given below. Cresols, hexachlorophene, alkyl- and chloro derivatives and diphenyls are more active than phenol itself. Physical methods of microbial control involve strategies that don't depend on chemicals, such as heating. Those articles which cannot withstand high temperature can still be sterilised at lower temperature by prolonging the duration of exposure. It is essential to fit the test tubes, flasks, etc. This pressure is measured at 15 pounds per square inch psi , or 1 atmosphere. Ultrasonic devices are used in dental and some medical offices to clear debris and saliva from instruments before sterilization and to clean dental restorations.

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Chemical Methods of Sterilization and Disinfection

physical methods of sterilization in microbiology

Disadvantages : Damages skin, eyes. It sometimes happens that all spores do not pass into vegetative forms before the second heating period. Convection currents travel a complete circuit through the wall space and interior of the oven, and the products of combustion escape through an opening in the top. Action is through attack on protein sulfhydryl groups and disruption of enzyme functions. Bacon, for example, can be sterilized by radappertization, a process of sterilizing foods by exposure to radiation, using radiation doses of 4. This steam condenses on the material and relieves the latent heat repeatedly to convert back into the water. The filtrate is collected in a glass tube inside the cup.

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Techniques of Sterilization in Bacteria

physical methods of sterilization in microbiology

However, moist-heat sterilization is typically the more effective protocol because it penetrates cells better than dry heat does. Filters simply remove contaminating microorganisms from solutions rather than directly destroying them. Boiling requires heating water to 212 degrees Fahrenheit for at least ten minutes. The solution is then pushed or forced through the filter with a vacuum or with pressure from a syringe, peristaltic pump, or nitrogen gas bottle, and collected in previously sterilized containers. A Streak of Bad Potluck One Monday in spring 2015, an Ohio woman began to experience blurred, double vision; difficulty swallowing; and drooping eyelids. Pasteurization : Fresh beverages such as milk, fruit juices,beer, and wine are easily contaminated during collection and processing.


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Sterilization » Microbiology Online Notes

physical methods of sterilization in microbiology

Ultraviolet sources are used in biological safety cabinets for partial contamination control and should be turned on only when cabinets or enclosures are not in use. By steam sterilization, these forms of bacteria are also killed as steam destroys the. They do not rupture during filtration. Such pressure—temperature combi-nations can be achieved only with a special device that can subject pure steam to pressures greater than 1 atmo-sphere. Lid is fastened by screw clamps and rendered air tight by an asbestos washer. The autoclave is still considered the most effective method of sterilization.

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Sterilization » Microbiology Online Notes

physical methods of sterilization in microbiology

The filtration equipment and the filters themselves may be purchased as presterilized disposable units in sealed packaging, or must be sterilized by the user, generally by autoclaving at a temperature that does not damage the fragile filter membranes. Thus, microbial control, or control of the growth of microbes, is necessary for preserving human health. Two other methods of pasteurization are the flash pasteurization method at 71. Physical Control by Other Methods: Filtration : Heat is valuable physical agent for controlling microorganisms but sometimes it is important to use. Chemical indicators should be positioned near the center of each load, and toward the bottom front of the autoclave.

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Sterilization » Microbiology Online Notes

physical methods of sterilization in microbiology

Desiccation : In addition to freezing, many foods are preserved by desiccation. If the temperature goes above 180°C. In determining the time and temperature for microbial destruction with heat, certain factors bear consideration. If wearing a suit, the air pressure within the suit must be higher than that outside the suit, so that if a leak in the suit occurs, laboratory air that may be contaminated cannot be drawn into the suit Figure 1. A convenient arrangement is shown in Fig. Thus, there is no risk for exposure to radioactive material through eating gamma-irradiated foods.

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